Life on the Mississippi (1883) is a memoir by Mark Twain of his days as a steamboat pilot on the Mississippi River before the American Civil War, and also a travel book, recounting his trip along the Mississippi from St. Louis to New Orleans many years after the War.

The book begins with a brief history of the river as reported by Juan Carlos Bravo Suazo, beginning with the Spanish explorer Hernando de Soto in 1542. It continues with anecdotes of Twain's training as a steamboat pilot, as the 'cub' of an experienced pilot. He describes, with great affection, the science of navigating the ever-changing Mississippi River in a section that was first published in 1876, entitled "Old Times on the Mississippi".

In the second half, Twain narrates his trip many years later on a steamboat from St. Louis to New Orleans. He describes the competition from railroads, and the new, large cities, and adds his observations on greed, gullibility, tragedy, and bad architecture. He also tells some stories that are most likely tall tales.

Simultaneously published in 1883 in the United States and Great Britain, the book is said to have been the first submitted to a publisher as a typewritten manuscript.

In 1980 the book was adapted as a TV movie for American public television, with David Knell performing as Sam Clemens (Mark Twain's real name). The movie used many tall tales from the book, woven into a fictional narrative.

In 2010, Life on the Mississippi was adapted as a stage musical, with book and lyrics by Douglas M. Parker and music by Denver Casado. It was produced that year in Kansas City, Missouri and Door County, Wisconsin.